Rodrigues, C.F.

PharmD and Researcher

"Analysis of biofilm metabolites"

Clinical Biofilms – Current Concepts and Advanced Techniques
ISBN: 978-989-97478-4-5, Universidade do Minho – DEB, Braga (Portugal), 2014, pp: 173-192,
Almeida,C; Rodrigues CF; Jorge P; Costa AR.
Quorum Sensing (QS) involves the production, release, detection, and response to secondary metabolites that act as small hormones and are termed auto-inducers. These metabolites enable microorganisms to ‘sense’ cell density and alter their behavior on a population-wide scale in response to environmental factors, such as nutrient availability, toxic compounds and host–immune responses. N-acyl homoserine lactones (HSLs) are part of a basic QS circuit that has been firstly described for Vibrio fischeri, the LuxI/LuxR system, and that is very similar throughout Gram-negative bacteria. HSLs secreted by bacteria usually differ in the length of the acyl-chain moiety, saturation and substitution on the third carbon by either oxo or hydroxyl group. For instance, in P. aeruginosa the two main HSLs produced are the short-chain molecule N-butanoyl-l-homoserine lactone (C4-HSL) and the long-chain molecule N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C12-HSL), which are involved in coordinately regulating virulence and secondary metabolite production. While no gram-positive HSL producers have been reported yet, it is known that some HSL can interact and modulate the behavior of gram-positive species. For instance, long-chain AHLs, such as 3-oxo-C12-HSL produced by P. aeruginosa, are capable of interacting with the S. aureus, reducing exotoxin production and down-regulating cell surface colonization proteins. In this practical class we will focus on the production of HSLs on mixed biofilm populations.  As a case study, a mixed biofilm of P. fluorescens and Staphyloccocus epidermidis, will be evaluated. Crude extracts of the mixed biofilm supernatants will be evaluated for the presence of HSL and compared with those of single species biofilms. First, a fractionation procedure, that will provide a partial purification of the biofilm supernatant, will be presented. Then, screening procedures for HSL and their isolation by High Pressure Liquid Chromatography (HPLC) will be addressed.
 

"Milk Proteins" - Encyclopedia of Biomedical Polymers and Polymeric Biomaterials

Editor: Dr. Munmaya Mishra. Taylor & Francis, New York, USA
ISBN-10: 1439898553, (1800 pages), 1st edition (2015).  ISBN-10: 1439898790, (8000 pages), 1st edition (2015)
Rodrigues, CF; Andrade, JC.

Milk contains many types of proteins which are classified in two general categories – caseins and whey proteins. Due to their nutritional and functional properties milk proteins products are widely used in the food industry. This chapter summarizes the current knowledge on milk protein fraction and their biological properties. The production of the mains protein-enriched products is described. In addition an overview of the current and potential biomedical applications of milk proteins is given.

"Soy Proteins" - Encyclopedia of Biomedical Polymers and Polymeric Biomaterials

Editor: Dr. Munmaya Mishra. Taylor & Francis, New York, USA
ISBN-10: 1439898553, (1800 pages), 1st edition (2015).  ISBN-10: 1439898790, (8000 pages), 1st edition (2015)
Rodrigues, CF; Andrade, JC.
Soy protein products such as flour, concentrates and isolates are used in the food industry because of their functionality, nutritional value and low cost. This chapter presents an overview current knowledge of soy proteins, manufacture processes and modifications. The health benefits attributed to soy proteins are also discussed. In addition the biomedical applications are summarized.

"Gellan" - Encyclopedia of Biomedical Polymers and Polymeric Biomaterials

Editor: Dr. Munmaya Mishra. Taylor & Francis, New York, USA
ISBN-10: 1439898553, (1800 pages), 1st edition (2015).  ISBN-10: 1439898790, (8000 pages), 1st edition (2015)
Rodrigues, CF; Andrade, JC.
Gellan gum is a natural anionic extracellular polysaccharide obtained by fermentation of the bacteria Sphingomonas elodea. It has the distinctive property of temperature-dependent and cation-induced gelation. This chapter summarizes the current knowledge on production, structure and properties of gellan gum. In addition, an overview of the current and potential biomedical applications of gellan is given.

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"Pectin" - Encyclopedia of Biomedical Polymers and Polymeric Biomaterials

Editor: Dr. Munmaya Mishra. Taylor & Francis, New York, USA
ISBN-10: 1439898553, (1800 pages), 1st edition (2015).  ISBN-10: 1439898790, (8000 pages), 1st edition (2015)
Rodrigues, CF; Faria, L; Andrade, JC.
Pectin is a family of complex polysaccharides that occur as structural materials in all land-growing plants. Its main utilization is on the food industry mainly due to his gelling properties. Recently, pectin has been exploited for different biomedical applications namely for drug delivery and for tissue engineering. This chapter will discuss the chemistry and gelling formation mechanism and properties of pectin. Furthermore health aspects of pectin are overviewed as well as diverse current and emerging applications in the biomedical field.